Germination requirements of Bacillus macerans spores.

The nutritional requirements for germination and outgrowth of spores and vegetative cell growth of some aerobic spore forming bacteria.

During the last 10 years the subject of bacterial spore germination has been intensively investigated. In most of these studies it has been found that bacterial spores are capable of germination under conditions which do not permit the germinated form to survive (Schmidt, 1955; Stedman, 1956). Hills (1949) pointed out that a distinction must be drawn between the requirements for germination and...

Effect of subtilin and nisin on the spores of Bacillus coagulans.

The early literature on the effect of the polypeptide antibiotics subtilin and nisin on bacterial spores has been reviewed by Campbell and O'Brien (1955). Most of the early reports have indicated that subtilin acts as a sporostatic agent. Other reports have shown, however, that the spores of several bacterial species will germinate in the presence of subtilin and are subsequently killed by the ...

Adenine and 2,6-diaminopurine as germinants for Bacillus macerans spores.

Superdormant spores of Bacillus species have elevated wet-heat resistance and temperature requirements for heat activation.

Purified superdormant spores of Bacillus cereus, B. megaterium, and B. subtilis isolated after optimal heat activation of dormant spores and subsequent germination with inosine, d-glucose, or l-valine, respectively, germinate very poorly with the original germinants used to remove dormant spores from spore populations, thus allowing isolation of the superdormant spores, and even with alternate ...

Metabolic requirements for microcycle sporogenesis of Bacillus megaterium.

Spores of Bacillus megaterium QM B1551 germinated, elongated, and resporulated (microcycle sporogenesis) in simple chemically defined media which permitted no cell division. The second-stage spores thus produced were heat-stable and required heat activation for germination. The original amount of spore deoxyribonucleic acid tripled before completion of the cycle. Acetate and a small amount of a...